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Affinity (AC)


Affinity Chromatography

WorkBeads Protein A

Affinity Chromatography

Affinity chromatography is a useful chromatography technique separating proteins in a reversible interaction between the target protein and ligand of bead. The interaction can be biospecific for example antibodies binding to protein A or non-biospecific for example histidine-tagged proteins binding to metal ions.


WorkBeads Protein A

WorkBeads Protein A affinity chromatography media is produced from agarose using a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose matrices have successfully been used for many years in biotechnology research and in the industrial purification of proteins.

The recombinant protein A attached to the medium is developed by Medicago and produced in E. coli under conditions free of components of animal origin. The protein A is engineered to facilitate an oriented coupling to the matrix which allows high binding capacities for target proteins. The specificity of the recombinant protein A for the Fc region of IgG provides excellent purification. The high capacity, chemical stability and low ligand leakage make WorkBeads Protein A ideal for purification of mono- and polyclonal antibodies.


 - WorkBeads Protein A
 

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