左側相關訊息
Sepax

SRT-C SEC 系列



- Particle size of 5 µm
- Selection of pore size: 100, 150, 300, 500, 1,000, and 2,000 Å
- High separation efficiency and resolution
- High capacity
- High lot-to-lot reproducibility
- High protein recovery with intact biological activity
- Negligible non-specific interactions

Column詳細資訊 *   產品資訊電子檔 * 
Sepax SRT-C SEC Columns

SRT®-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. SRT®-C, uses lay-down monolayer on porous silica as oppose to SRT of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation.

Along with the widest pore size selection of 100, 150, 300, 500, 1000 and 2000Å, SRT-C SEC packings have highest capacity that enables highest resolution.
 

Lay-down 技術




 


Specifications
 
Phase SRT-C SEC-100 SRT-C SEC-150 SRT-C SEC-300
Material Neutral, hydrophilic film bonded silica
Particle size 5 µm
Pore size ~ 100 Å ~ 150 Å ~ 300 Å
Protein MW range (native) 100 - 100,000 500 - 150,000 5,000 - 1,250,000
pH stability 2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure
(psi for a 7.8×300 mm)
~ 700 ~ 700 ~ 700
Maximum backpressure (psi) ~ 4,500 ~ 4,500 ~ 3,500
Salt concentration range 20 mM - 2.0 M
Maximum temperature (°C) ~ 80
Mobile phase compatibility Aqueous and organic
 

 

 

 

 

 

 















 

Phase SRT-C SEC-500 SRT-C SEC-1000 SRT-C SEC-2000
Material Neutral, hydrophilic film bonded silica
Particle size 5 µm
Pore size ~ 500 Å ~ 1,000 Å ~2,000 Å
Protein MW range (native) 15,000 - 5,000,000 50,000 - 7,500,000 > 10,000,000
pH stability 2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure
(psi for a 7.8×300 mm)
~ 700 ~ 700 ~ 700
Maximum backpressure (psi) ~ 3,000 ~ 3,000 ~ 3,500
Salt concentration range 20 mM - 2.0 M
Maximum temperature (°C) ~ 80
Mobile phase compatibility Aqueous and organic





 

 

 

 

 

 

 

 

 









*Sepax also offers custom-made columns.
 Both stainless stell and PEEK tubes are available.

 
 





 

 

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