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Bio-Works

Ion Exchange (IEX)


WorkBeads 40 Q and S
WorkBeads DEAE


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WorkBeads 40 Q and S

WorkBeads™ 40 Q and WorkBeads™ 40 S have a high selectivity which means the protein peaks are well separated with greater distance from each other than comparable products made from synthetic polymers. This means that the media has capacity to separate proteins well even when using high proteins loadings.

Resolution is the combined effect of selectivity (distance between peaks) and efficiency (peak width, depending on particle size). Separation media based on agarose are well known for excellent selectivity. The particle size of 40 micrometer with an very narrow particle size distribution in combination with the proprietary cross linking results in columns packed with very high efficiency and excellent flow characteristics well suited to demanding bioprocess applications.


 - WorkBeads 40 Q and S
 



WorkBeads DEAE

WorkBeads™ 40 DEAE ion exchange media are produced from agarose using a proprietary cross linking method that results in a highly porous and physically stable agarose matrix. Agarose based matrices have been successfully used for decades in biotechnology research and in the industrial purification of proteins. Agarose is proven to be exceptionally compatible with natural biomolecules like proteins, DNA, carbohydrates etc. The material shows minimal non specific interaction due to the hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the microenvironment in the column which lead to distorted separations.

WorkBeads 40 DEAE have a high selectivity which means the protein peaks are well separated with greater distance from each other than comparable products made from synthetic polymers. This means that the media has capacity to separate proteins well even when using high proteins loadings.
DEAE is a weak ion exchanger meaning that above pH 9 and higher there will be very few ion exchange groups facilitating CIP. The charge density is also lower that WorkBeads 40 Q which will give a different selectivity.


 - WorkBeads DEAE
 

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