左側相關訊息
其他各式廠牌管柱

PolyLC



PolyCAT A™
- for Cation-Exchange of Proteins -
1) Protein variants involving deamidation, PEGylation, position of attachment, desialylation, etc. It is widely used by the biotechnology industry.
2) Hemoglobin variant analysis by clinical chemistry labs.
3) Proteins with pI above 6.0 (5.0 in special cases).
4) Monoclonal antibodies.
5) Histones.

PolyETHYL A™ / PolyMETHYL A™ / PolyPROPYL A™
- for HIC of Proteins and Peptides -
1) Multidimensional protein purification (suggested sequence: Ion-exchange - [add salt to collected fractions] - HIC).
2) Purification of polypeptides (e.g., venoms and glycopeptides).
3) Characterization of antibodies.
4) QC analysis of proteins differing in the polarity of a single residue or modified positional variants.


PolyGLYCOPLEX A™
- for HILIC of Complex Carbohydrates -
Selectivity is good for both native glycans and derivatives such as those with the 2-aminopyridine (PA-) fluorophore. Oligosaccharide mixtures can often be resolved isocratically, although gradients are recommended for especially varied samples. Sialylated and asialo- glycans can be resolved using the same running conditions.  In some cases selectivity compares favorably with that of HPAEC with PAD detection, and both running conditions and equipment maintenance are far more convenient.

PolyHYDROXYETHYL A™

- for Hydrophilic Interaction and Size Exclusion HPLC -
1) Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma or crude extracts.
2) Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).
3) Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.
4) HPLC of solutes that aren't soluble in aqueous media (membrane proteins;phospholipids).
5) Eliminating detergents, lipids, and salts from a sample.
6) Oligonucleotides and their analogs.


​- for Cation-Exchange of Peptides-
PolySULFOETHYL A™
1) Multidimensional HPLC of peptide mixtures, such as tryptic  digests in proteomics analyses (including iTRAQ® and ICAT®* reaction products).
2) Isolation of peptides from natural products.
3) QC and purification of synthetic peptides.
4) Selective isolation of disulfide-linked peptides, phosphopeptides and C-terminal fragments from tryptic digests.
5) Mapping of peptide digests (tryptic, V8, CNBr, etc.).

PolyWAX LP™


- for Anion-Exchange of Proteins and Nucleic Acids -
1) Purification of acidic proteins and polypeptides from natural products.
2) Analysis and purification of oligonucleotides and their analogs as well as amplified PCR products.
3) In proteomics, predigest fractionation of intact proteins via mixed-bed ion-exchange.
4) Anion-exchange of small, acidic solutes.

PolySAX LP™

 

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